The Mississippi Flyway is a bird migration route that generally follows the Mississippi River in the United States and the Mackenzie River in Canada. The main endpoints of the flyway include central Canada and the region surrounding the Gulf of Mexico. The migration route tends to narrow considerably in the lower Mississippi River valley in the states of Missouri, Arkansas, Mississippi, and Louisiana, which accounts for the high number of bird species found in those areas. Some birds use this flyway to migrate from the Arctic Ocean to Patagonia.

Typically birds use this route because no mountains or ridges of hills block the path over its entire extent. Good sources of water, food, and cover exist over its entire length. About 40% of all North American migrating waterfowl and shorebirds use this route.